Dikarya - Phylogenetic Tree Builder

Phylogenetic Tree Builder

Add sequences to build a phylogenetic tree with advanced analysis options.

Import from iNaturalist

iNaturalist Observation URL

Paste an iNaturalist observation URL to import DNA Barcode ITS sequences.

Import from Mycomap

Mycomap BLAST Results URL

Paste a Mycomap BLAST result URL to import sequences.

NCBI Results Local Results Remove Duplicates

BLAST for Related Sequences

Enter Single Sequence or Accession

Find related sequences on NCBI GenBank.

Min Identity (%)

Max Results

Or Upload a File

Click to upload or drag and drop

FASTA files (.fa, .fasta, .fna, .txt)

Job Name / Notes

Add Custom Sequences

Paste FASTA Sequences

Enter sequences in FASTA format.

Sequence Queue

0 sequences

No sequences added yet.

Paste FASTA sequences and click "Add Sequences"

Click × to remove

2. Pipeline Configuration

Alignment Method

Choose the algorithm for multiple sequence alignment.

Trimming Method

Remove poorly aligned regions to improve signal.

Tree Inference

Method

Substitution Model

E.g., GTR+G, JC69, HKY.

Quick Tips

BLAST: Enter a single sequence or GenBank accession to find related sequences
Quick Tree: Uses MAFFT + FastTree 2.2.0 (Maximum Likelihood)
Advanced: Choose alignment, trimming, and tree methods