Phylogenetic Tree Builder

iNaturalist Observation URL or ID

Enter a 7 to 9 digit iNaturalist observation number or paste a full observation URL.

Mycomap BLAST Results URL

Paste a Mycomap BLAST result URL to import sequences.

NCBI Results Local Results Remove Duplicates

Enter Single Sequence or Accession

Find related sequences on NCBI GenBank.

Min Identity (%)

Max Results

Click to upload or drag and drop

FASTA files (.fa, .fasta, .fna, .txt)

Job Name / Notes

Paste FASTA Sequences or GenBank Accessions

Enter FASTA sequences or one or more GenBank accessions.

No sequences added yet.

Paste FASTA sequences and click "Add / Remove Sequences"

Pipeline: MAFFT → FastTree · GTR+CAT · 1000 BS · midpoint root

Import MycoMap results to enable filtering by query coverage, subject coverage, and identity.

Advanced

Currently: MAFFT + FastTree 2.2.0, midpoint-rooted, 1000 bootstrap.

Alignment Method

Choose the algorithm for multiple sequence alignment.

Trimming Method

Remove poorly aligned regions to improve signal.

Method

Substitution Model

E.g., GTR+G, JC69, HKY.