Dikarya - Phylogenetic Tree Builder

Import from iNaturalist

iNaturalist Observation URL or ID or username or project

Enter an observation ID, observation URL, username, user URL, project name, or project URL.

Import from Mushroom Observer

Mushroom Observer Observation URL or Number

Current, short, and legacy Mushroom Observer observation links are supported.

Import from Mycomap

Mycomap BLAST Results URL

Paste a Mycomap BLAST result URL to import sequences.

NCBI Results Local Results Remove Duplicates Filter Conflicting Local FASTA Keep Exact Matches at Different Locations

BLAST for Related Sequences

Enter Single Sequence or Accession

Find related sequences on NCBI GenBank.

Min Identity (%)

Max Results

Or Upload a File

Click to upload or drag and drop

FASTA files (.fa, .fasta, .fna, .txt)

Job Name / Notes

Add Custom Sequences

Paste FASTA Sequences or GenBank Accessions

Enter FASTA sequences or one or more GenBank accessions.

Sequence queue

No sequences added yet.

Paste FASTA sequences and click "Add / Remove Sequences"

Pipeline: MAFFT → FastTree · GTR+CAT · 1000 BS · midpoint root

Import MycoMap results to enable filtering by query coverage, subject coverage, and identity.

Advanced

Pipeline Configuration

Currently: MAFFT + FastTree 2.2.0, terminal overhang trim, midpoint-rooted, 1000 bootstrap.

Alignment Method

Choose the algorithm for multiple sequence alignment.

Trimming Method

Remove poorly aligned regions to improve signal.

Trim terminal overhangs Remove low-coverage alignment ends before tree building.

Tree Inference

Program

Substitution Model

E.g., GTR+G, JC69, HKY.

Phylogenetic Tree Builder

Import from iNaturalist

Provisional Species Names: