| name |
Limacellopsis sp-Burnett-5-xi-1994 |
| author |
Tulloss |
| name status |
cryptonomen temporarium |
| GenBank nos. |
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|
| intro |
Olive text indicates a specimen that has not been
thoroughly examined (for example, for microscopic details) and marks other places in the text
where data is missing or uncertain.
The following material is based upon original research by R. E. Tulloss.
|
| pileus |
??, white to pale cream with pink tint, ??, eventually irregularly planar; context ??; margin nonstriate, incurved at first, ??; gluten layer present. |
| lamellae |
??, close, white or whitish; lamellulae ??. |
| stipe |
??, dry; bulb subglobose and distinct in exsiccata; context solid (distinct central cylinder filled with sordid tissue in exsiccata); partial veil superior, white, skirt-like, membranous, at first infundibuliform, retaining sinus at stipe after drooping (as, e.g., in L. guttata), ??; gluten layer ??. |
| odor/taste |
not recorded. |
macrochemical
tests |
none recorded |
| pileipellis |
absent |
| lamella trama |
bilateral; central stratum distinct, comprising interwoven filamentous undifferentiated hyphae (?? µm wide) with frequent fusiform intercalary segments (?? × ?? µm ); ??. |
| basidia |
26–30 × 6.3–7.8 µm, 4-sterigmate, with sterigmata up to 3.8 × 1.5 µm; clamps prominent, plentiful. |
| gluten layer |
On pileus: filamentous hyphae supporting gluten pile 1.7–6.0 µm wide, branching, gelatinizing extensively in older material; terminal cells approximately conic to pyriform to fusiform to constricted-subfusiform to "sausage-shaped," occasionally capitulate, frequently rostrate, 12.5–49 × 4.0–13.5 µm, 2.0–5.5 µm wide at base, with length/max.-width ratio = 2.1–6.0, occasionally with pale yellow vacuolar pigment, erect at first, then collapsing without dominant orientation and gelatinizing, arising either from uninflated or slightly inflated hyphal segments or from subglobose to broadly ellipsoid inflated cells (10.8–42 × 9.5–33 µm, apparently sometimes in short chains); clamps common. On stipe: ??. |
| stipe context |
longitudinally acrophysalidic; filamentous undifferentiated hyphae 1.9–8.5 µm, dominating, branching; acrophysalides 40–92± × 7.8–20 µm; vascular hyphae not observed??; clamps common. |
| lamella edge tissue |
fertile |
| basidiospores |
RET from proposed epitype:
[60/3/1] (4.1–) 4.3–6.0 (–7.2) × (3.2–) 3.4–4.6 (–5.5)
µm, (L = 5.0–5.2 µm; L’ = 5.2 µm;
W = 3.9–4.2 µm; W’ = 4.0 µm; Q =
(1.06–) 1.09–1.53 (–1.94); Q = 1.26–132;
Q’ = 1.30), hyaline, colorless, smooth,
dominantly inamyloid, occasionally dextrinoid, broadly
ellipsoid to ellipsoid, infrequently subglobose,
rarely elongate, adaxially flattened; apiculus
sublateral, cylindric; content granular to mono- to
multiguttulate; color in deposit not recorded. |
| ecology |
In small groups. |
| material examined |
U.S.A.:
WASHINGTON—Whatcom Co. - Lummi Isl.,
5.xi.1994 Nancy Burnett s.n. (RET 136-9, nrITS & nrLSU
seq'd.). |
| discussion |
This collection matches the original description of L. roseicremea except for the size and shape of the spores. However, the few spores that I was able to find on a surviving paratype of L. roseicremea are a good match to the spores of the present fungus.
For purposes of comparison, the available spore data (red figure) from the type of L. roseicremea is given here: [8/1/1] 4.5 – 6.2 × 3.5 – 4.5 µm, (L = 4.8 µm; L’ = 4.8 µm; W = 3.7 µm; W’ = 3.7 µm; Q = (1.21–) 1.22 – 1.43; Q = 1.30; Q’ = 1.30).
For purposes of comparison, RET's spore data (green figure) from European material of L. guttata is given here: [12/1/1] 4.8 - 5.8 (-6.1) × (3.9-) 4.0 - 4.3 (-5.0) µm, (L = 5.3 µm; W = 4.1 µm; Q = 1.20 - 1.42; Q' = 1.30).
For purposes of comparison, the very limited available spore data (orange figure) of L. solidipes is given here: [-/-/-] 4 – 5 × 4 – 5 µm, (est. Q = 1.0 – 1.25). [Nota: This data should not be presumed to be reliable.—ed.]
The comparison of sporographs (above) indicates that, in the present state of knowledge, spore size and shape do not provide a sufficient data set for segregation the taxa reported from North America that are similar to L. roseicremea. Hence, more detailed microscopic studies, including revision of all relevant types will be necessary to develop clear species concepts in this group of taxa within Limacella sect. Amanitellae. It is possible that some of the names will prove to be taxonomic synonyms.
The terminal cells of the gluten supporting hyphae in the pileal gluten layer conform very closely to the terminal cells in the corresponding tissue of L. guttata; as described by Neville and Poumarat (2004). Moreover, a persistent, membranous partial veil is clearly present. Therefore, it is proposed that the present species should be considered to belong in Limacella subsect. Amanitellae. |
| citations |
—R. E. Tulloss |
| editors |
RET |
|
Spore data sets and their composite