| name |
Amanita sp-M12 |
| author |
Tulloss |
| name status |
cryptonomen temporarium |
| GenBank nos. |
Due to delays in data processing at GenBank, some accession numbers may lead to unreleased (pending) pages.
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|
| intro |
Olive text indicates a specimen that has not been thoroughly examined (for example, for microscopic details) and marks other places in the text where data is missing or uncertain.
The following material is based upon original research by R. E. Tulloss. |
| pileus |
31 - 57 mm wide, usually some range of olivaceous tints, uniformly colored or distinctly or indistinctly zonate (e.g., with pale [nearly cream] disc or brown without orange tint [e.g., 7D4 over umbo] in center of disc and yellowish olivaceous for remainder of disc, sometimes with more or less brown zone over inner ends or entirety of marginal striations), occasionally entirely brownish gray (Tulloss 8-29-94-D), not changing when cut or bruised, occasionally (Tulloss 8-29-94-G) subvirgate, campanulate at first, then broadly convext to subconvex with sometimes flattened disc region including low or distinct umbo, tacky to dry, dull to subshiny; context white, sometimes with faint brownish gray tint just under pileipellis, 2 - 4.5 mm thick, thinning evenly for 0.5R to 0.8R toward margin, then membranous; margin striate (0.3R - 0.5R), decurved, nonappendiculate; universal veil absent or (Tulloss 8-29-94-D) as scattered membranous patches, white or pallid. |
| lamellae |
free, almost always without decurrent line on stipe apex, but occasionally with faint such line, crowded, pale cream to faintly yellowish cream in mass, white in side view, unchanging when cut or bruised, 3 - 6.5 mm broad, broadest at 0.5 to 0.8 distance from stipe to margin, occasionally anastomosing, sometimes with white fimbriate edge; lamellulae truncate to subattenuate, unevenly distributed, of diverse lengths, common to plentiful, occasionally isolated from both margin and stipe (Tulloss 8-29-94-G). |
| stipe |
45 - 65 × 4 - 9 mm, pale cream to off-white, occasionally with brownish longitudinal lines, occasionally with brownish pruina, sometimes becoming sordid or faintly brown from handling, faintly longitudinally striatulate (lens), with short pallid fibrils or pulverulence on upper third or less, cylindric or narrowing upward or downward, at first not or just barely flaring at apex, distinctly flaring at apex in age; context white, with watery brownish longitudinal streaks or faintly brownish near exterior surface, unchanging when cut or bruised, stuffed with fine white fibrils making up tissue of variable density (from loosely interwoven fibrils to densely packed and vertically arranged fibrils) or hollow and lined with white cottony fibrils (occasionally forming crosswalls near stipe base), with central cylinder 1.5 - 6 mm wide, with larva tunnels concolorous; exannulate; universal veil as saccate volva, exterior surface white with rare to scattered faint to distinct brown stains, interior white with orange-brown dots, soft, membranous, subcylindric, with irregular and sometimes partially fragmented limbs, 17 - 32 × 7.5 - 18 mm, < 1 mm thick, attached low on stipe, with distinct (but proportionately small) limbus internus slightly above point of attachment. |
| odor/taste |
Odor indistinct. Taste not recorded. |
macrochemical
tests |
Spot test for laccase (syringaldazine) - negative throughout basidiome. Spot test for tyrosinase (paracresol) - ??. Test voucher: Tulloss 8-29-94-C. |
| pileipellis |
35 - 60 µm thick; suprapellis pallid to colorless, sometimes very reduced, extensively gelatinized, 0+ - 20 µm thick; subpellis orange-brown, 35 - 45 µm thick, comprising partially gelatinized to ungelatinized elements; filamentous, undifferentiated hyphae 2.0 - 5.5 µm wide, criss-crossed singly or (more commonly) in broad fascicles, occasionally having yellowish subrefractive walls, occasionally containing refractive yellow-brown to orange-brown to brown guttules or nonguttulate regions; vascular hyphae 3.0 - 6.4 µm wide, sinuous, occasionally coiling, scattered, infrequent. |
| basidia |
38 - 57 × 10.2 - 14.5 µm, dominantly 4-, also rather commonly 2-, and rarely 3- or 1-sterigmate, with thin to slightly thickened walls, with sterigmata up to 6.8 × 3.8 µm; clamps not observed. |
| partial veil |
absent. |
| basidiospores |
[120/6/5] (7.5-) 8.8 - 11.5 (-15.5) × (6.3-) 7.5 - 10.0 (- 14.3) μm, (L = 9.8 - 10.1 (-10.3) μm; L' = 10.0 μm; W = (8.3-) 8.,4 - 9.0 μm; W' = 8.7 μm; Q = (1.04-) 1.06 - 1.27 (-1.62); Q = (1.11-) 1.15 - 1.18; Q' = 1.16), hyaline, colorless, smooth, thin-walled, inamyloid, with variable shape (subglobose to broadly ellipsoid, infrequently globose, rarely elongate, occasionally sublangeniform or sublachrimiform, infrequently contorted-langeniform, sometimes slightly constricted), sometimes expanded at one end, adaxially flattened; apiculus sublateral to (infrequently subapical), cylindric, proportionately broad; contents dominantly monoguttulate, infrequently to commonly multiguttulate. |
| ecology |
Solitary to paired (once subcepitose), at 2600± m elev. In moist dark loam and oak litter (above brown tepetate) of woods dominated by Quercus spp. but also including Juniperus deppeana Steud. and with understory including Helianthemum glomeratum ??. |
| material examined |
MÉXICO: TLAXCALA—Mpio.
Panotla - 1 km E of San Francisco Temezontla
[19°20’41” N/ 98°16’31” W, 2640 m], 29.viii.1994 A.
Montoya Esquivel, A. Kong Luz, & R. E. Tulloss
[Tulloss 8-29-94-C] (RET 135-3, nrITS seq'd.;
TLXM), [Tulloss
8-29-94-D] (RET 135-4; TLXM), [Tulloss 8-29-94-G]
(RET 134-9; TLXM), [Tulloss 8-29-94-H] (RET 135-2,
nrITS seq'd.;
TLXM), [Tulloss 8-29-94-I] (RET 135-1, nrITS seq'd.;
TLXM),
15.vii.1996 A. Montoya Esquivel
& R. E. Tulloss [Tulloss 7-15-96-L] (RET 253-9;
TLXM), [Tulloss 7-15-96-M] (RET 253-7, nrITS & nrLSU
seq'd.; TLXM). |
| discussion |
The present species may be rather closely related phenetically to the subarctic and alpine species A. nivalis Grev. known from specimens examined [by RET] from Europe and Greenland and reported from northeastern Asia. Characters that A. sp-M12 has in common with the more northern species are
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subhymenial tree predominantly a branching structure of uninflated or partially inflated hyphal segments
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pileipellis thin, with minimal suprapellis, and comprising criss-crossing filamentous, undifferentiated hyphae
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spores that are rather variable in size and shape on a single basidiome, rather commonly misshapen, having wide range of Q
-
....more??....
|
| citations |
—R. E. Tulloss |
| editors |
RET |
|
Spore data sets and their composite
