| name |
Amanita aurorae |
| name status |
cryptonomen temporarium |
| english name |
double click in markup mode to edit. |
| GenBank nos. |
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|
| intro |
Olive text indicates a specimen that
has not been thoroughly examined (for example, for
microscopic details) and marks other places in the text
where data is missing or uncertain.
The following material is derived from photographs and
notes of the collector, molecular research of Dr. L. V.
Kudzma and other original research of R. E. Tulloss. |
| odor/taste |
neither recorded. |
macrochemical
tests |
none recorded. |
| partial veil |
not present. |
| lamella edge tissue |
sterile. |
| ecology |
Ontario, Canada: In mixed woods with
Quercus rubra,
Acer saccharum, and Betula
papyrifera.
Florida, U.S.A.: In office park landscaping
with mostly
Quercus and Lagerstroemia.
Texas, U.S.A.: In sandy soil of forest including
Quercus and Pinus. |
| material examined |
CANADA:
ONTARIO—Port Cover, lynn Valley Rd. &
Haldimand Rd. 70 [42.8380° N/ 80.1211° W, 201 m],
30 viii.2018 Eva Skivic s.n.
[mushroomobserver
#330370]
(RET 841-5, nrITS-LSU seq'd.).
Roseneath, Peter's Woods
Prov. Nat. Reserve, 15.vii.2014 Jan Thornhill s.n.
(RET 637-3, nrITS & nrLSU seq'd.).
U.S.A.:
FLORIDA—Leon Co. - Tallahassee
[30.4264º N/ 84.2847º W, 62 m],15.vi.2017 Russell
de Grove 2-092106 (RET 782-8, nrITS-LSU seq'd.),
22.vi.2017 R. de Grove s.n. [mushroomobserver
#279764]
(RET 783-7, nrITS-LSU seq'd.); unkn. loc., 15.vi.2017
Russell de Grove 3-151449-092128 (RET 782-4, nrITS-LSU
seq'd.); unkn. loc., 15.vi.2017 Russell de Grove
4-145103 (RET 782-9), nrITS-LSU seq'd.).
TEXAS—Tyler
Co. - Big Thicket Nat. Preserve - North Turkey Creek
Unit [30°36.164’ N/ 94°20.913’ W, 45.1 m], 11.vi.2015 Ronald L.
Pastorino 6-11-14K [mushroomobserver
#168799]
(RET 629-2, nrITS & nrLSU seq'd.).
WISCONSIN—Walworth Co., Lulu Lake
St. Natural Area [42.8362° N/ 88.4525° W, 252 m],
2.ix.2018 Andrew Khitsun s.n. [mushroomobserver
#331731]
(RET 850-7, nrITS-LSU seq'd.). |
| discussion |
Molecular observations
Stephen Russell has reported an additional collection
of the present species from Indiana
(https://mycomap.com/9697).
A comparison of the nrITS gene from four collections
(RET 629-2, RET 637-3, RET 850-7, and Mycomap 9697)
was made by alignment of the sequences.
In addition to the
sequence segment posted by Stephen, Karen Hughes
(Univ. Tenn., Knoxville) and Linas Kudzma also produced
relevant sequences. If we look at all the data,
we have data extending from within the ITS1F primer
site in nrSSU into nrLSU. The overall aligned
region is 746 characters long.
The 5' terminus of nrITS falls at character position
76.
The 3' terminus of nrITS falls at character
position 577.
Mycomap 9697 runs between character
positions 29 and 661 and
includes the entire nrITS.
A rough characterization of the sequences based on
the alignment follows:
-
char. 143 is a G in the RET 629-2 data, but
an A in the other sets of data.
-
At around char. 248-250 there is a multi-"A"
repeat. The length differs by up to two
characters. This could occur naturally or in
the process of copying the DNA for sequencing.
It is a difference without any weight to it.
Mycomap 9697 is distinguished by having a "T" at
position 248 prior to the repeat.
-
char. 277 is a C in RET 629-2, RET 850-7,
and Mycomap 9697 while in
the other data there is a
clear T (the raw data sometimes shows a T combined
with a
C as a distinctly lesser peak). Maybe a SNP?
-
The same is true for char. 288.
-
Char. 304 is an A in RET 629-2, RET
850-7, and Mycomap 9697. It is a G in the other
data.
That is the complete list of differences.
Summary: At minimum, there are two 2 invariant
differences and
two possible differences (if the SNP suggestions
don't hold up to further examination).
The Ontario material (RET 637-3) was briefly treated
on the present site as "Amanita
sp-pseudocrocea02." The species has been more
recently
treated under the names "A. sp-F20" and
"A. sp-T47." |
| citations |
—R. E. Tulloss and Linas Kudzma |
| editors |
RET |
|
Spore data sets and their composite